激發(fā)光源用于觀(guān)察荔枝愈傷的GFP表達

近期中國熱帶農業(yè)科學(xué)院在《Horticultural Plant Journal》期刊上發(fā)表了題為《Agrobacterium tumefaciens-mediated transformation of embryogenic callus and CRISPR/Cas9-mediated genome editing in ‘Feizixiao’ litchi》文獻，文獻中采用了LUYOR-3415RG便攜式雙波長(cháng)激發(fā)光源用于觀(guān)察GFP綠色熒光蛋白在荔枝愈傷上的表達。

LUYOR-3415RG便攜式雙波長(cháng)激發(fā)光源照射荔枝愈傷,有綠色熒光蛋白的發(fā)出綠色熒光

文獻摘要：

The embryogenic callus was transformed under optimum transformation conditions. After the second round of Hyg screening, resistant yellow callus in 31.58% of the total transformed callus was selected and half was used to induce somatic embryos. Finally, histochemical staining of callus and somatic embryos for GUS gene expression determination and a hand-held lamp (LUYOR 3415RG, Shanghai, China) were used to investigate the expression and resulting fluorescence signals of GFP in resistant transgenic callus and somatic embryos.

文獻地址：https://doi.org/10.1016/j.hpj.2023.01.011   

LUYOR-3415RG雙波長(cháng)熒光蛋白激發(fā)光源

LUYOR-3415RG手持式熒光蛋白激發(fā)光源可檢測轉GFP、eGFP、tdtomato、cherry、dsred、dapi、hoechst、cfp、bfp、yfp、venus、lucife等多種熒光蛋白的表達。激發(fā)光源激發(fā)出綠色熒光時(shí)，用黃色濾光鏡觀(guān)測，可檢測含綠色熒光蛋白（GFP）的生物；激發(fā)光源激發(fā)紅色熒光時(shí)，用紅色濾光鏡觀(guān)測，可檢測含紅色熒光蛋白（DsRed）的生物。LUYOR-3415RG雙熒光蛋白激發(fā)光源用于檢測、篩選轉綠色熒光蛋白（GFP）和紅色熒光蛋白（DsRed）基因的植物、動(dòng)物及微生物，如水稻、玉米、斑馬魚(yú)、小鼠、細菌、真菌等等, LUYOR-3415RG和LUYOR-3430、LUYOR-3260除了可用于篩選轉基因生物外，還可以用于樣本的預篩選、輔助解剖、用于珊瑚研究等，

產(chǎn)品介紹：LUYOR-3415RG雙波長(cháng)便攜式熒光蛋白激發(fā)光源

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